✅ Board Important Q&A
Q. What is applied microbiology? What is industrial microbiology? What are its main features?
Applied Microbiology: Branch of biology that studies enzymes, proteins, applied genetics and molecular biology of prokaryotes and eukaryotic microbes for producing food and medicines on large scale for society.
Industrial Microbiology: Science related to commercial use of microbes for various economic, social and environmental processes and products.
Main features: (A) Various productions through fermentation — bread, cheese, wine, raw material for chemicals, enzymes, nutrients, medicines. (B) Use of microbes for garbage management and pollution control.
Q. Explain the process of yoghurt production.
Yoghurt is produced with help of lactobacilli (inoculants). Milk is boiled, then cooled to warm temperature. Two bacterial strains are added in 1:1 proportion — Streptococcus thermophilus and Lactobacillus delbrueckii.
Streptococcus produces lactic acid → proteins gel out → gives dense consistency to yoghurt.
Lactobacilli produce acetaldehyde compounds → gives characteristic taste.
Condensed milk powder is added to maintain protein content. Pasteurization improves shelf life and probiotic properties.
Q. How does bread become spongy?
Baker's yeast (Saccharomyces cerevisiae) is mixed with flour, water, salt and other materials to form dough. Yeast performs fermentation of carbohydrates: Sugar → CO₂ + Ethanol. The CO₂ gas produced gets trapped in the dough and makes it rise. During baking, gas expands further and ethanol evaporates. This gives the bread its spongy, porous texture. Yeast also makes bread nutritious as it contains carbohydrates, fats, proteins, vitamins and minerals.
Q. What are probiotics? Why are they important?
Probiotics: Milk products containing active live bacteria like Lactobacillus Acidophilus, Lactobacillus casei, Bifidobacterium bifidum. Available as yoghurt, kefir, sauerkraut, dark chocolate, pickles, etc.
Importance: (1) Maintain balance of intestinal microorganisms — increase helpful microbes and decrease harmful ones (Clostridium). (2) Improve resistance and lower ill-effects of harmful metabolic substances. (3) Reactivate useful microbes made inactive by antibiotics. (4) Used for treatment of diarrhoea and in poultry treatment. (5) Form colonies of useful microbes in alimentary canal.
Q. Why are microbial enzymes better than chemical catalysts in industry?
(1) Active at low temperature, pH and pressure — saves energy and no need for erosion-proof instruments. (2) Enzymes carry out specific processes — no unnecessary by-products formed, reducing purification costs. (3) Waste material elimination and decomposition avoided — enzymes can be reused. (4) Eco-friendly — do not cause pollution. These advantages make microbial enzymes preferred over chemical catalysts in chemical, cheese, textile, leather and paper industries.
Q. How do microbes help in cleaning oil spills? What is bioremediation?
Petroleum oil spilling in ocean is toxic and fatal to aquatic organisms. It cannot be easily removed by mechanical methods. Hydrocarbonoclastic bacteria (HCB) like Pseudomonas spp. and Alcanovorax borkumensis can destroy pyridines and other hydrocarbons. They decompose the hydrocarbons and react carbon with oxygen, forming CO₂ and water. These bacteria are used to clean oil spills in oceans.
Bioremediation: Absorption or destruction of toxic chemicals and harmful pollutants using microorganisms or plants (phyto-remediation). Dr. Anand Mohan Chakravarti (India-born American scientist) first suggested use of such microbes for oil spill cleaning.
Q. What are biofuels? What are the fuels obtained through microbial processes?
Biofuel: Renewable fuel obtained from biological sources. Available in solid (coal, dung, crop residue), liquid (vegetable oils, alcohol) and gaseous (biogas, coal gas) forms.
Fuels from microbes:
(1) Methane — microbial anaerobic decomposition of urban, agricultural and industrial waste (biogas plants).
(2) Ethanol — fermentation of molasses by Saccharomyces. Clean, smokeless fuel. Mixed with petrol/diesel to reduce pollution.
(3) Hydrogen — bio-photolysis of water in which bacteria perform photoreduction. Considered fuel of the future.